RELATIONSHIP BETWEEN BCL-2 EXPRESSION AND APOPTOSIS INDEX ON RAT (RATTUS NORVEGICUS) MODEL OF PREECLAMPSIA AFTER ADMINISTRATION OF EVOO

Abstract

One cause of preeclampsia is due to an imbalance between antioxidants and free radicals, due to the failure of spiral artery remodeling, resulting in placenta ischemia and hypoxia. The occurring hypoxia mediates mir210 to induce the expression of the antiapoptotic Bcl-2 (Bcell lymphoma-2) target gene that may reduce excessive apoptosis. This study aims to prevent this imbalance by giving extra virgin olive oil (EVOO) rich in tocopherol content (vitamin E). The design of this study is pre- and post-test with control group design in the laboratory. Subject is a female rat, Sprague Dawley strain, weight ± 200g. The control and treatment groups consisted of a total sample of 25 individuals. Bcl-2 expression increased significantly, especially in the treatment group that received EVOO (P value 0,009). Apoptosis index tended to decrease in treatment group that received low and moderate dose of EVOO, but there was no significance (P value 0.332). Conclusions: EVOO was able to increase Bcl-2 to prevent excessive apoptosis on preeclampsia. It is recommended to examine the expression of p53 or Bax protein to find out which protein regulates apoptotic.