ACUTE TOXICITY TEST AND HISTOLOGICAL DESCRIPTION OF ORGANS AFTER GIVING NANO HERBAL ANDALIMAN (Zanthoxylum acanthopodium)

Abstract

Andaliman is a typical plant (Northen Sumatera, Indonesia) contains antioxidants and as herbal medicine in the future. This aimed in this research to determine the toxic value of nano herbal and aliman and analyze histology of the liver, lungs, heart and brain after given of this herb. Nano herbal andaliman were made using High Energy Milling (HEM). LC50 was used Brine Shrimp Lethality Test with concentrations of 10, 100, 1000, 10000 ppm while LD50 consists of 3 stages: dose orientation test (12 mice), preliminary test (20 mice) and LD50 determination (30 mice) for 14 today by the Thomson Weil formula, then the organ was investigated for histology. The results of this research: LC50 value=1737.80 ppm, LD50 =9.807g/KgBW ± 0.075 and a significant difference was noticed (p <0.05) in liver weight but no significant difference (p> 0.05) on the weight of lungs, heart and brain. A significant difference also in organ histology on the administration nano herbal andaliman in each dose level. Nano herbal andaliman belongs to the category of mild toxic so that it could be processed as a herbal medicine of the future.